Isolation and Characterization of a cDNA Clone Encoding One IgE-Binding Fragment of <i>Penicillium brevicompactum</i>
Bibliographic record
Abstract
<i>Background:</i>The abundance of allergenic <i>Penicillium</i> species has been associated with an increased incidence of childhood asthma and pulmonary bleeding. <i>Penicillium brevicompactum </i>has been identified as the most prevalent indoorspecies of this genus. However, detailed studies on the allergens of the ubiquitous <i>Penicillium </i>species are still lacking. For the characterization of allergens of prevalent <i>Penicillium </i>species, molecular cloning of the allergen genes of <i>P. brevicompactum</i> was performed in the present study. <i>Methods:</i>A phage cDNA library of <i>P. brevicompactum</i> was constructed in Uni-ZAP<sup>®</sup> XR vector using mRNA isolated from the organism. The cDNA library of <i>P.</i><i>brevicompactum</i> was screened using pooled atopic sera. <i>Results:</i> Screening of <i>P. brevicompactum</i> cDNA library resulted in one positive clone encoding an estimated molecular weight of 11 kDa polypeptide, rich in acidic residues (>20%), with a pI of 3.87. This clone was designated as <i>Pen b </i>26 and found to be reactive only against the atopic sera obtained from individuals sensitive to <i>P. brevicompactum</i>. The amino acid sequence analysis of <i>Pen b </i>26 revealed that it had strong homology to the 60S acidic ribosomal protein P1 family from different eukaryotic sources, predominantly fungal aero-allergens. Other features of <i>Pen b </i>26 including having high alpha-helical content (>50%), alanine-rich residues (>20%), and a well-conserved C-terminal epitope region fits well into the common properties of 60S acidic ribosomal proteins. <i>Conclusions:</i>The results obtained suggest that the allergenic clone, <i>Pen b </i>26 is a 60S acidic ribosomal protein P1 of <i>P. brevicompactum</i> and shows strong similarity to other P1 family proteins.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".