Abstract P278: Identification of Differentially Expressed Aortic Genes in Brown Norway Introgressed Chromosome 2 Segments into Hypertensive Dahl Salt Sensitive Rats
Bibliographic record
Abstract
Background: Rat chromosome (RNO) 2 introgression from normotensive Brown Norway (BN) rats into hypertensive Dahl salt sensitive (SS) background (consomic SB2) reduced vascular inflammation. We hypothesized that the BN-RNO2 contains genes that reduce vascular inflammation, which could be identified using microRNA (miRNA) and total RNA expression profiling in aorta of congenic rats containing different portions of BN-RNO2 on the SS background. Methods and Results: Twelve-to-13-week-old male SS rats and congenic rats containing the distal portion of BN-RNO2 (SB2a), the middle segment (SB2b) and the proximal segment (SB2e) on the SS background, fed a normal-salt diet, were studied. Systolic blood pressure (SBP) was measured by telemetry. SBP was lower in SB2a and SB2b but not SB2e compared to SS (125±3, 127±6, 138±4 vs 146±2 mm Hg, P <0.05). Total RNA was extracted from aorta and used to construct libraries for small and total RNA sequencing using Illumina HiSeq-2500. The bioinformatics pipeline included: FastQC for quality control, STAR for genome alignment to Rattus norvegicus release-86, mirdeep2 for miRNA annotation and counting, Htseq-count for mRNA and long non-coding RNA annotation and counting; R for differential expression analysis. Differentially expressed miRNAs and genes (mRNA and non-coding RNA) were identified in SB2a vs SS (miRNAs: 3 up and 2 down, genes: 1 up and 3 down), SB2b vs SS (miRNAs: 2 up and 3 down, genes: 67 up and 112 down) and SB2e vs SS (miRNAs: 29 up and 25 down, genes: 12 up and 35 down), with FDR<0.05. Differentially expressed genes encoded within different BN-RNO2 congenic portions were identified in SB2a vs SS (2 down), SB2b vs SS (14 up and 18 down) and SB2e vs SS (1 down). Conclusions and Perspectives: Differentially expressed BN-RNO2 encoded genes were identified in aorta of congenic SB2a, SB2b and SB2e rats. Whether these genes play a role in inflammation or vascular injury remains to be determined.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".