OC-093 The Effects of Faecal Microbiota Transplantation on the Innate Immune System and Epithelial Tight Junction Expression in Chronic Refractory Pouchitis
Bibliographic record
Abstract
Introduction Faecal microbiota transplantation (FMT) may be beneficial in IBD. A durable change in recipients’ microbiota following FMT has been demonstrated. No previous study has assessed the immunological effects of FMT. We aimed to assess the immunological effects of FMT in patients with chronic refractory pouchitis. Methods FMT was performed via nasogastric tube. Mucosal biopsies samples were collected at pouchoscopy from eight patients with chronic refractory pouchitis before and four weeks after FMT. The epithelium was identified following incubation with EDTA and lamina propria dendritic cells (DCs) were identified following collagenase digestion. Epithelial cells were identified as pancytokeritin positive cells and expression of ZO-1, claudin 1 and claudin 2 were measured by multicolour flow cytometry. DC were identified as an HLA DR+, lineage- (CD3-,CD14-,CD16-,CD19-,CD34-) population. Expression of TLR 2, 4 and 5, β7 and CCR 9, and CD40 were measured by multicolour flow cytometry. Cytokines were assessed by multiplex ELISA of biopsy supernatants. The t-test was used in statistical analysis. Results There was a tendency for increased expression of claudin 2 (18.6% +- 11.4% v 39.6% +-13.4%, p = 0.3). Lower levels in lamina propria DC expression of TLR4(45.8% +- 6.5% v 33.6% +- 6.9%) and TLR 5(35.5% +-12.2% v 23.3% +-11.5%) as well as β7 expression (39.9% +-7.6% v 31.9% +-4.5%) were not statistically significant. There were no changes in the levels of IL2, 4, 6, 10, TNF, IFN or IL17 in the supernatant of mucosal biopsies before or 4 weeks after FMT. However, a rise in IL10 (9.1pg/ml +-1.2 v 12.5pg/ml +-2.9, p = 0.2) was noted following FMT. Conclusion There were no significant alterations in epithelial tight junction expression or immunological factors studied following FMT. In chronic refractory pouchitis, faecal transplantation may not be effective in altering the aberrant immunological response. Disclosure of Interest None Declared
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".