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Record W4254641809 · doi:10.22215/etd/2014-10514

Ghrelin Signaling Within the Paraventricular Nucleus of the Hypothalamus Influences Sympathetic Activity But Not Brown Adipose Tissue Activity During the Stress Response

2014· dissertation· en· W4254641809 on OpenAlexaff
Tamara Parno

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldMedicine
TopicAdipose Tissue and Metabolism
Canadian institutionsCarleton University
Fundersnot available
KeywordsBrown adipose tissueEndocrinologyInternal medicineHypothalamusThermogenesisGhrelinThermogeninStimulationNorepinephrineAdipose tissueEnergy homeostasisReceptorBiologySympathetic nervous systemMedicineDopamine

Abstract

fetched live from OpenAlex

In mammals, ghrelin is secreted during chronic stress to promote the utilization of carbohydrates while decreasing the use of fat as a source for energy.Data suggest that ghrelin secretion during stress may also regulate energy expenditure in the form of heat derived from the activation of brown adipose tissue (BAT).Activation of BAT involves the sympathetic stimulation of non-shivering thermogenesis by the action of uncoupling protein-1 (UCP-1).Furthermore, the paraventricular nucleus of the hypothalamus (PVN) plays a role in energy expenditure by reducing sympathetic outflow on to BAT, thereby decreasing UCP-1 expression, and ultimately decreasing heat production.To do this, mice were implanted with cannulae attached to osmotic minipumps delivering a ghrelin receptor antagonist [D-Lys-3]-GHRP6 (20nmol/day/mouse) or vehicle by the PVN.Half of the mice from each group were subjected to chronic social defeat stress for 19-21 days.Results indicated that stressed animals decreased UCP-1 mRNA expression within BAT, although there were no drug effects.Stressed animals given the antagonist showed increased plasma epinephrine (EP) and norepinephrine (NE) compared to mice in the other groups, but appeared to show less utilization of these in BAT.We therefore suggest that stress alters sympathetic tone to modulate the expression of UCP-1 in BAT and that these effects are not mediated by ghrelin acting on receptors in the PVN.make me feel confident in my work and he always believed in my intelligence, even when I never did.I could not have asked for a better thesis supervisor and I am eternally grateful for that.I would also like to thank the rest of my committee, Iain McKinnell, Mike Hildebrand, Shawn Hayley, and John Stead for allowing me to create the best version of my thesis.I would like to especially thank Zack Patterson for introducing me into graduate work.I worked with him during my undergraduate thesis and he suggested I join the Abizaid community.He was my mentor and I always thought of myself as a hard worker until I met Zack.He's moving away from the Abizaid lab but I'm just grateful I met him for that brief time.I would also like to

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.269
Teacher spread0.255 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes1
Has abstractyes

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