Molecular Genetics of Self-incompatibility in Brassica napus
Bibliographic record
Abstract
Self-incompatibility (SI) is one of the mechanisms evolved by higher plants to promote outbreeding. In Brassica species, SI is controlled sporophytically by a single Mendelian genetic locus, the S-locus. Pollen rejection or acceptance is determined by the parental genotype, and pollen grains carrying the same allele as that present in the stigma are rejected. Among the genes identified so far from different S-haplotypes, the S-locus glycoprotein (SLG) and the S-locus receptor kinase (SRK) have been extensively studied. The current model of SI in Brassica postulates that a highly polymorphic pollen-borne ligand would be specifically recognized by SLG and/or SRK. This would activate a signal transduction pathway leading to rejection of self-pollen. We used a genomic approach in an attempt to identify a putative ligand gene. The S-locus regions of two functional Brassica napus S-haplotypes (910 and A14) were subcloned in Fosmid vectors and sequenced. New transcription units were identified by homology searches in the databases, gene prediction programs and polymerase chain reaction (PCR) amplification. The current study reports the structural features of both S-locus regions and a functional analysis using a transformation-based assay.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".