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Record W4281572412 · doi:10.1038/s42003-022-03453-2

The Pel polysaccharide is predominantly composed of a dimeric repeat of α-1,4 linked galactosamine and N-acetylgalactosamine

2022· article· en· W4281572412 on OpenAlexafffund
François Le Mauff, Erum Razvi, Courtney Reichhardt, Piyanka Sivarajah, Matthew R. Parsek, P. Lynne Howell, Donald C. Sheppard

Bibliographic record

VenueCommunications Biology · 2022
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBacterial biofilms and quorum sensing
Canadian institutionsSickKids FoundationUniversity of TorontoMcGill UniversityHospital for Sick ChildrenInstitute of Infection and ImmunityMcGill University Health Centre
FundersCIHR Skin Research Training CentreNational Institute of Allergy and Infectious DiseasesCanadian Glycomics NetworkU.S. Department of Health and Human ServicesGovernment of CanadaNational Institutes of HealthCanadian Institutes of Health ResearchMcGill University Health CentreMcGill University
KeywordsGalactosaminePolysaccharideHydrolysisChemistryStrain (injury)BiofilmBacteriaGlycoside hydrolaseBiochemistryPseudomonas aeruginosaMicrobiologyAcetylationBiologyGeneGalactoseGenetics

Abstract

fetched live from OpenAlex

Abstract The genetic capacity to synthesize the biofilm matrix exopolysaccharide Pel is widespread among Gram-negative and Gram-positive bacteria. However, its exact chemical structure has been challenging to determine. Using a Pseudomonas aeruginosa strain engineered to overproduce Pel, improvements to the isolation procedure, and selective hydrolysis with the glycoside hydrolase PelA h , we demonstrate that Pel is a partially de- N- acetylated linear polymer of α-1,4- N- acetylgalactosamine comprised predominantly of dimeric repeats of galactosamine and N- acetylgalactosamine.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.264
Threshold uncertainty score0.359

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.268
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations65
Published2022
Admission routes2
Has abstractyes

Explore more

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