Green Photosynthetic Microalgae from Low pH Environments Associated with Mining as a Potential Source of Antioxidants
Bibliographic record
Abstract
A potential commercial market for microalgal-produced antioxidants is a natural alternative to synthetic compounds that are possible carcinogens. Further, utilizing microalgae for carbon (CO2) capture from industrial off-gas could be environmentally beneficial for their mass production, but due to an increase in acidity of the growing media caused by acid gasses, microalgae able to survive at pH 3.0–4.0 while still producing antioxidant metabolites are needed. Two strains of green microalgae were bioprospected from acid mine drainage impacted water bodies (pH 2.9) in Canada. These and a culture collection strain Chlamydomonas reinhardtii (pH 7.0) were investigated for their antioxidant capacity and chlorophyll content while growing under low pH conditions. The isolates were identified as Coccomyxa sp. and Chlamydomonas sp. based on ITS sequences. The microalgae were grown at pH 3.0 and unregulated pH media for 28 d and their antioxidant potential evaluated with three complimentary assays. The results showed that C. reinhardtii did not grow at pH 3.0, and that Coccomyxa sp. had significantly higher antioxidant potential than Chlamydomonas sp. Both species also showed significantly higher antioxidant potential than C. reinhardtii when it was grown at pH 7.0.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".