Isolation and Expression Analysis of <i>BvNHX1</i> from <i>Beta vulgaris</i> with High Sucrose
Bibliographic record
Abstract
In present study, the vacuolar Na + /H + exchanger gene was isolated by homologous cloning technology from the high-sucrose Beta vulgaris (‘BS02’), referred as BvNHX1 , which contained an ORF of 1 659 bp, encoded 552 amino acids, the protein molecular weight was 61.31 kD, and the theoretical isoelectric point was 6.31. The protein encoded by BvNHX1 gene had 12 transmembrane domains and the conserved domains of Nhap, Na_H_Exchanger and b_cpa1 superfamily, and grouped with various NHXs of Chenopodiaceae plants, such as Salicornia europaea , Atriplex dimorphostegia , Suaeda salsa , and belonged to Class I in the vacuolar Na + /H + exchanger family. Under 400 mmol/L NaCl, 200 mmol/L KCl and 15 mmol/L ABA, the expression of BvNHX1 reached the peaks in leaves and roots, respectively, and the expression of BvNHX1 in leaves was significantly higher than that in roots, indicating that the expression of BvNHX1 was induced by NaCl, KCl and ABA, and it may play a greater role in leaves than roots in response to abiotic stresses. This study will lay a foundation for the study of the salt tolerance molecular mechanism in Beta vulgaris with high sucrose, and provide a solid and reliable basis for the genetic improvement of salt tolerance in Beta vulgaris with high sucrose.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".