Cloning and Expression Analysis of <i>NAC2</i> Gene of Potato (<i>Solanum tuberosum L</i>)
Bibliographic record
Abstract
NAC is one of the unique transcription factor families in plants, which plays an important regulatory role in response to abiotic stress. In this study, a NAC transcription factor gene StNAC2 was cloned in potato ‘Yunshu 505’ by RT-PCR. Sequence analysis showed that the ORF of StNAC2 gene was 876 bp, encoding 291 amino acids, including a conserved Nam domain with a molecular weight of 33.585 12 kD, a theoretical isoelectric point of 7.04, an instability coefficient of 50.33 and an average hydrophilic number of -0.660. Protein prediction results showed that StNAC2 protein is an unstable hydrophilic protein with no signal peptide and transmembrane domain, which can be expressed in both cytoplasm and mitochondria. Homology comparison and phylogenetic tree analysis showed that StNAC2 gene is closely related to Capsicum annuum and Nicotiana tab acum. Spatio-temporal expression results showed that the expression of StNAC2 gene was different in tissue, the expression of StNAC2 gene was higher in leaves than in stems. The expression of StNAC2 gene was induced under cadmium stress, and reached the maximum value at 50 mg/kg, but decreased significantly after being treated with 100 mg/kg cadmium, indicating that the expression of StNAC2 gene was inhibited under high concentration stress and StNAC2 gene could involve in cadmium stress. The results lay the foundation for dissecting the molecular mechanism of StNAC2 gene involved in Cd stress response.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".