Identification And Functional Analysis of 3-Phosphatidylinositol Kinase <i>FAB1/PIKfyve</i> Gene Family in Rice (<i>Oryza sativa</i>)
Bibliographic record
Abstract
FAB1/PIKfyve is a key enzyme that catalyzes phosphatidylinositol 3-phosphate (PtdIns3P) to form phosphatidylinositol 3,5-bisphosphate (PtdIns (3,5) P 2 ). Its product PtdIns (3,5) P 2 plays an important role in the development of eukaryotic cells. In order to figure out the function of PtdIns (3,5) P 2 in rice reproductive development, this study combined bioinformatics and genetics methods to identify the rice FAB1/PIKfyve genes, analyzed the physical and chemical properties, gene structures, conserved domains, phylogenetic tree, cis-acting elements, tissue expression profiles and used CRISPR/Cas9 gene editing technology to obtain osfab1b mutants. Bioinformatics analysis results showed that nine FAB1 gene family members were identified from the whole genome of Oryza Sativa . Gene structure analysis indicated the differences existed in the gene structure of FAB1 family, the number of exons are 8-12. Conserve domain analysis showed that OsFAB1A and OsFAB1B contained the N-terminal FYVE domain, and the remaining members contained the Cpn60_TCP1 domain and PIPKc kinase domain. Phylogenetic analysis indicated that the functions of FAB1 family were highly conserved in mono- and dicotyledonous plants. Prediction of cis-element in the upstream regulatory region of FAB1 genes revealed a variety of growth-related, light-responsive, and hormone and stress-responsive cis-elements. Tissue expression profiles showed that most of FAB1 genes were global-expressed, and the high expression of FAB1C sub-cluster genes in lemma and palea suggested that they might be involved in floral organ development. Finally, the osfab1b mutants were obtained through the CRISPR/Cas9 system, and the pollen vitality of the osfab1b mutants showed no significantly abnormality, implied that FAB1 family had functional redundancy in the reproductive development of rice. The present results provide a theoretical reference for biological function studies of the phosphatidylinositol regulatory network in rice.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".