Analysis of MYB Transcription Factor Family Based on Transcriptome Sequencing in Colored Potato
Bibliographic record
Abstract
MYB transcription factor family is one of the transcription factors with various functions and numerous quantities, which plays an important role in plant growth and metabolism regulation. In this study, based on the transcriptome sequencing data of color potato tubers, we screened MYB transcription factor genes during the development of color potato, and analyzed its conservative domain and subcellular localization; meanwhile, we analyzed the differential expression of R2R3 MYB transcription factor by TMEV software, and predicted the function of R2R3 MYB transcription factor with significant difference. The results showed that based on the transcriptome sequencing (RNA-Seq) data, 143 transcription factors of MYB family were annotated, selected, and divided into four categories (1R-MYB, R2R3-MYB, 3R-MYB and 4R-MYB) according to their structural characteristics. The result of the subcellular localization demonstrated that 138 MYB transcription factors were located in the nucleus, one MYB transcription factors were located in the cytoplasm, and four MYB transcription factors were located in the cell membrane. Conservative domain analysis showed that the conserved motifs of R2R3-MYB transcription factors are R2 and R3. Colored potato R2R3-MYB transcription factor gene was differentially expressed in different periods, among which 10 were up-regulated and 5 were down-regulated. According to the analysis of GO enrichment, 6 transcription factors of R2R3-MYB related to anthocyanin were annotated. This study will provide a reference for further study on the biological function and metabolic regulation mechanism of MYB gene related to anthocyanin synthesis in color potato.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".