Cloning and Expression Analysis of <I>atp6</i> Genes between Cytoplasmic Male Sterile Line and Its Maintainer Line in Bunching Onion
Bibliographic record
Abstract
In order to further study the relationship between atp6 gene and Cytoplasm male sterility (CMS) of Bunching onion, the sequence of atp6 gene was obtained from male sterile line and maintainer line of Zhangqiu onion bunching using homologous cloning. The protein secondary structure and the expression of atp6 gene in different growth stages were compared. The results showed that there was one polymorphic locus (A/T) at the 171st base of the open reading frame of atp6 gene in CMS line and maintainer line, which changed the proportion of protein secondary structure slightly, but did not change the transmembrane structure of ATP6 protein. The expression of atp6 gene in roots and leaves/flowers at seedling, bolting, flowering and late flowering stage was detected by real-time fluorescence quantitative polymerase chain reaction. The results showed that the expression of atp6 gene in root showed a trend of increasing at first and then decreasing during the whole development of the plant, reaching the maximum at the flowering stage, and the expression of the maintainer line was 1.14 times higher than that of the CMS; The expression of atp6 gene in leaves/flowers decreased gradually during the whole growth period, and reached the highest level at the seedling stage, and the maintainer line was 1.96 times higher than that of the CMS.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".