Transcriptome Response of Cannabis ( <i>Cannabis sativa</i> L.) to the Pathogenic fungus <i>Golovinomyces ambrosiae</i>
Bibliographic record
Abstract
Abstract Powdery mildew (PM), caused by the obligate biotrophic fungus Golovinomyces ambrosiae , is an economically important fungal disease of hemp - and marijuana–type cannabis. While the PM disease can be managed effectively by cultivating resistant hosts, there is no known PM-resistant genetic variant. This is the first report of transcript level responses of the hemp cultivar ‘X59’ to G. ambrosiae . Transcript level changes at 5-, 8-, and 11-days post-inoculation (DPI) of C. sativa were evaluated against uninoculated control. Our analysis revealed that 1,898 genes were significantly ( q -value < 0.05) differentially expressed (DE) following the pathogen challenge. Among these, 910 and 988 genes were upregulated and downregulated, respectively as the infection progressed to 11 DPI. Genes related to salicylic acid (SA), (LOC115715124 and LOC115711424) and WRKY transcription factor (LOC115707546, LOC115715968, and LOC115707511) were highly upregulated. There were 45 DEGs that were homologous to PM-related genes, including chitin elicitor receptor kinase 1 ( CERK 1 ), enhanced disease resistance 2, ( EDR2 ), and powdery mildew resistance ( PMR ) genes. Moreover, the genes related to glycosyl hydrolases, particularly LOC115699396, LOC115708023, LOC115710105, and LOC115710100, were highly upregulated and potentially important in mediating pathogen responses. Collectively, this study has contributed to an enhanced understanding of the molecular mechanisms that are involved in cannabis and PM disease interaction and has identified several gene candidates that can be further investigated for their role in defence mechanisms.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".