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Record W4291016130 · doi:10.1093/ve/veac050

Recurrent SARS-CoV-2 mutations in immunodeficient patients

2022· article· en· W4291016130 on OpenAlexfundno aff
Samuel Wilkinson, Alex Richter, Anna Casey, Husam Osman, Jeremy Mirza, Joanne Stockton, Josh Quick, Liz Ratcliffe, Natalie Sparks, Nicola Cumley, Radoslaw Poplawski, Beatrix Kele, Kathryn Harris, Thomas P. Peacock, Nicholas J. Loman

Bibliographic record

VenueVirus Evolution · 2022
Typearticle
Languageen
FieldMedicine
TopicSARS-CoV-2 and COVID-19 Research
Canadian institutionsnot available
FundersMedical Research CouncilSheffield Teaching Hospitals NHS Foundation TrustQuadram Institute BioscienceUniversity College London Hospitals NHS Foundation TrustNewcastle upon Tyne Hospitals NHS Foundation TrustQueen's University BelfastPublic Health EnglandRoyal Marsden NHS Foundation TrustSwansea UniversityUniversity of BrightonUniversity of OxfordUniversity of SouthamptonUniversity College LondonDirectorate for Biological SciencesImperial College LondonUniversity of St AndrewsQueen's UniversityUniversity of East AngliaNational Institute for Health and Care ResearchPublic Health AgencyRoyal Free London NHS Foundation TrustUniversity Hospital Southampton NHS Foundation TrustKing's College Hospital NHS Foundation TrustUK Research and InnovationKing's College LondonUniversity of PortsmouthUniversity of ExeterNorthumbria UniversityPublic Health WalesMiddlesex University
KeywordsMutationBiologyGeneGeneticsVirusVirologyComputational biology

Abstract

fetched live from OpenAlex

Abstract Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections in immunodeficient patients are an important source of variation for the virus but are understudied. Many case studies have been published which describe one or a small number of long-term infected individuals but no study has combined these sequences into a cohesive dataset. This work aims to rectify this and study the genomics of this patient group through a combination of literature searches as well as identifying new case series directly from the COVID-19 Genomics UK (COG-UK) dataset. The spike gene receptor-binding domain and N-terminal domain (NTD) were identified as mutation hotspots. Numerous mutations associated with variants of concern were observed to emerge recurrently. Additionally a mutation in the envelope gene, T30I was determined to be the second most frequent recurrently occurring mutation arising in persistent infections. A high proportion of recurrent mutations in immunodeficient individuals are associated with ACE2 affinity, immune escape, or viral packaging optimisation. There is an apparent selective pressure for mutations that aid cell–cell transmission within the host or persistence which are often different from mutations that aid inter-host transmission, although the fact that multiple recurrent de novo mutations are considered defining for variants of concern strongly indicates that this potential source of novel variants should not be discounted.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.002
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.044
GPT teacher head0.345
Teacher spread0.301 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations137
Published2022
Admission routes1
Has abstractyes

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