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Record W4292527439 · doi:10.47068/ctns.2022.v11i21.037

NEW THERMOPHILIC THERMOBIFIDA STRAIN KB-T3 FROM ALGERIAN SAHARAN SOIL: ISOLATION AND POLYPHASIC TAXONOMY

2022· article· en· W4292527439 on OpenAlexaff
Nacera Benoussaid, Dalila Boubetra, Affaf Laassami, Khaoula Bouznada, Michael J. Holtz, Noureddine Bouras, Atika Meklat

Bibliographic record

VenueCURRENT TRENDS IN NATURAL SCIENCES · 2022
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsAgriculture Food and Rural Development
FundersNational Eye InstituteNational Brain Research Centre
Keywords16S ribosomal RNABiologyMyceliumXyloseStrain (injury)ThermophileMicrobiologyBotanyBacteriaBiochemistryFermentationGenetics

Abstract

fetched live from OpenAlex

During a screening for the diversity of actinobacterial strains from Saharan soil samples collected from Béchar region (Algeria), one strain designated KB-T3 was isolated by dilution technique on chitin-vitamins agar medium. The taxonomic position of this strain was determined by using a polyphasic approach. Morphological and chemical characteristics of the KB-T3 strain were consistent with those of the genus Thermobifida. The KB-T3 strain had a white aerial mycelium with dictomically branched sporophores carrying coccoid secluded spores. The substrate mycelium was pale yellow, sterile, and non-fragmented. The strain is characterized by the presence of meso-diaminopimelic acid in the cell wall, the galactose in whole-cell, and phosphatidylethanolamine in the cell membrane. The unique characteristic of this strain was its abundant growth with the absence of NaCl and in temperature‎ ranging from 40 to 65 °C, its capacity to decompose acetate, and its ability to use fructose, glucose and xylose as sole carbon source. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain KB-T3 should be classified in the genus Thermobifida and exhibited 99.79 % gene sequence similarity to Thermobifida fusca NBRC 14071T.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.837
Threshold uncertainty score0.483

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.029
GPT teacher head0.275
Teacher spread0.247 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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Same venueCURRENT TRENDS IN NATURAL SCIENCESSame topicGenomics and Phylogenetic StudiesFrench-language works237,207