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Abstract IA004: Mesenchymal progenitors and sarcomagenesis

2022· article· en· W4295926274 on OpenAlexaffabout
T. Michael Underhill

Bibliographic record

VenueClinical Cancer Research · 2022
Typearticle
Languageen
FieldMedicine
TopicSarcoma Diagnosis and Treatment
Canadian institutionsUniversity of British Columbia
Fundersnot available
KeywordsMesenchymal stem cellBiologyMalignancyProgenitor cellSynovial sarcomaCancer researchOncogeneGene expression profilingSarcomaPathologyCellGene expressionCell biologyStem cellMedicineGeneCell cycleGenetics

Abstract

fetched live from OpenAlex

Abstract Synovial sarcoma (SS) is an aggressive soft-tissue malignancy that can arise in any area of the body, and most frequently affects adolescents and young adults. The 10-year survival rate has been estimated at 50%. SS is characterized by a pathognomonic t(X;18)(p11.2;q11.2) translocation which produces a fusion oncogene named SS18-SSX. In mouse models, expression of SS18-SSX is sufficient to drive the development of SS-like tumors. Despite recent advancements in our understanding of SS biology, the cell of origin remains undefined. In most instances, it is likely mesenchymal in nature. We previously generated a mesenchymal progenitor (MP) specific Cre line that has enabled us to identify MPs within the embryo [MOU1], and characterize and define their lineage trajectories through development into the adult. This line has been used to generate a unique mouse model of SS where the human SS18-SSX fusion oncogene with an EGFP marker is conditionally expressed in adult MPs. This model shows 100% penetrant SS tumor formation with a median latency period of 16 weeks. Histologic analyses of tumors reveal characteristic cellular morphology and the expression of common markers of SS. Single-cell (sc) RNA-seq gene expression profiles of murine tumors recapitulate those observed in human SS samples and highlight genetic programs involved in sarcomagenesis. Using this model, we can identify and isolate neoplastic lesions prior to the development of grossly visible tumors and trace these cells through the complete transformation continuum. By comparing tumor cells to normal MPs, we aim to better understand the mechanisms underlying the SS18-SSX induced transformation of MPs. Together, this model identifies a cell of origin for SS and provides us with a unique opportunity to address the molecular and cellular drivers of SS. Citation Format: T. Michael Underhill. Mesenchymal progenitors and sarcomagenesis [abstract]. In: Proceedings of the AACR Special Conference: Sarcomas; 2022 May 9-12; Montreal, QC, Canada. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(18_Suppl):Abstract nr IA004.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.012

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.318
GPT teacher head0.550
Teacher spread0.232 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes2
Has abstractyes

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