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Record W4297373452 · doi:10.1016/j.celrep.2022.111412

A nuclear cAMP microdomain suppresses tumor growth by Hippo pathway inactivation

2022· article· en· W4297373452 on OpenAlexafffund
M. Dróżdż, Ashley S. Doane, Rached Alkallas, Garrett Desman, Rohan Bareja, Michael Reilly, Jakyung Bang, Maftuna Yusupova, Jaewon You, Zuhal Eraslan, Jenny Wang, Akanksha Verma, Kelsey Aguirre, Elsbeth Kane, Ian R. Watson, Olivier Elemento, Elena Piskounova, Taha Merghoub, Jonathan H. Zippin

Bibliographic record

VenueCell Reports · 2022
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicHippo pathway signaling and YAP/TAZ
Canadian institutionsMcGill UniversityMcGill Genome Centre
FundersJanssen PharmaceuticalsInstitute of Population and Public HealthNational Institute of Arthritis and Musculoskeletal and Skin DiseasesWeill Cornell Medical CollegeNational Institutes of HealthFeldstein Medical FoundationEmerson CollectiveSwim Across AmericaCanadian Institutes of Health ResearchCompute CanadaUniversity of California, Los AngelesLudwig Institute for Cancer ResearchNational Cancer InstituteMelanoma Research AllianceAmerican Skin AssociationBreast Cancer Research FoundationPfizerParker Institute for Cancer ImmunotherapyEli Lilly and CompanyArray BioPharma
KeywordsLipid microdomainCell biologyHippo signaling pathwayChemistrySignal transductionBiologyCancer researchBiochemistry

Abstract

fetched live from OpenAlex

Cyclic AMP (cAMP) signaling is localized to multiple spatially distinct microdomains, but the role of cAMP microdomains in cancer cell biology is poorly understood. Here, we present a tunable genetic system that allows us to activate cAMP signaling in specific microdomains. We uncover a nuclear cAMP microdomain that activates a tumor-suppressive pathway in a broad range of cancers by inhibiting YAP, a key effector protein of the Hippo pathway, inside the nucleus. We show that nuclear cAMP induces a LATS-dependent pathway leading to phosphorylation of nuclear YAP solely at serine 397 and export of YAP from the nucleus with no change in YAP protein stability. Thus, nuclear cAMP inhibition of nuclear YAP is distinct from other known mechanisms of Hippo regulation. Pharmacologic targeting of specific cAMP microdomains remains an untapped therapeutic approach for cancer; thus, drugs directed at the nuclear cAMP microdomain may provide avenues for the treatment of cancer.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.279
Threshold uncertainty score0.706

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.004
GPT teacher head0.184
Teacher spread0.180 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations25
Published2022
Admission routes2
Has abstractyes

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