Abstracts of the 15th International Symposium on bioluminescence and chemiluminescence
Bibliographic record
Abstract
ATP is the major source for metabolic energy in the cell and affects numerous cellular processes.Generally ATP is measured by luminescence assay using firefly luciferin-luciferase reaction as total ATP content in a mass of cells.We developed a luminescence microscope to measure ATP at the single cell level and applied it to monitor cytosolic ATP level throughout the apoptotic process in a HeLa cell transfected luciferase gene.As a result, apoptotic stimuli by four medicines (staurosporine, carbonyl cyanide p-trifluoro-methoxyphenylhydrazone, cycloheximide and actinomycin D) induced elevation of the ATP level after 1-2 h.This result confirmed previous data reported by luminometric assay.However, we observed a phenomenon just before cell death that some cells emit flash luminescence after 4-8 h stimulus.It is uncertain whether or not the flash luminescence is due to increased cytosolic ATP; such a result can not be obtained by conventional luminometric assay.The bioluminescence imaging assay at the cellular level would make a new research field, not only in ATP measurement but also in intracellular signal transduction, regulation of gene expression and other areas. Comparison of effects of uranium and americium on bioluminescent bacteria
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.002 |
| Meta-epidemiology (narrow) | 0.003 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.002 |
| Bibliometrics | 0.004 | 0.002 |
| Science and technology studies | 0.001 | 0.002 |
| Scholarly communication | 0.005 | 0.003 |
| Open science | 0.002 | 0.002 |
| Research integrity | 0.005 | 0.006 |
| Insufficient payload (model declined to judge) | 0.114 | 0.075 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".