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Record W4300415526 · doi:10.32942/osf.io/s5rd7

Genomic architecture of resistance and tolerance to Swiss needle cast and Rhabdocline needle cast diseases in Douglas-fir

2022· preprint· en· W4300415526 on OpenAlexafffund
Pooja Singh, Joanna St. Clair, Brandon M. Lind, Richard Cronn, Nicholas Wilhelmi, Mengmeng Lu, Dragana Obreht Vidaković, Richard C. Hamelin, David C. Shaw, Sally N. Aitken

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsUniversity of British ColumbiaUniversity of Calgary
FundersNatural Sciences and Engineering Research Council of CanadaGenome AlbertaAlberta Innovates Bio SolutionsGenome British ColumbiaUniversity of AlbertaAlberta InnovatesGénome QuébecCompute CanadaGenome Canada
KeywordsGenome-wide association studyBiologyDouglas firGenetic architectureSingle-nucleotide polymorphismJasmonic acidGeneCandidate genePlant disease resistanceResistance (ecology)GeneticsBotanyQuantitative trait locusGenotypeEcology

Abstract

fetched live from OpenAlex

Understanding the genetic architecture of tolerance and resistance to pathogens is important to monitor and maintain resilient tree populations. Here we investigate the genetic basis of tolerance and resistance and to needle cast disease in Douglas-fir (Pseudotsuga menziesii) caused by two fungal pathogens: Swiss needle cast (SNC) caused by Nothophaeocryptopus gaeumannii, and Rhabdocline needle cast (RNC) caused by Rhabdocline pseudotsugae). We performed a case-control genome-wide association analysis (GWAS) and found these traits to be polygenic. Significant associations with SNC resistance were found for SNPs in genes for stomatal regulation and ethylene and jasmonic acid pathways, which are known for their roles in plant defense and immunity. Top-associated SNPs for SNC tolerance were found in genes of secondary metabolite pathways. We identified a key upstream transcription factor of plant defence, ERF1, as the main candidate for RNC resistance. Our findings contribute to the understanding of the highly polygenic architectures underlying disease resistance and tolerance in Douglas-fir and have important implications for forestry and conservation as the climate changes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.235
Teacher spread0.227 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2022
Admission routes2
Has abstractyes

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