MétaCan
Menu
← Back to cohort
Record W4302011955 · doi:10.32920/ryerson.14668452.v1

The Fc receptor supramolecular complex from RAW macrophages

2022· preprint· en· W4302011955 on OpenAlexaff
Veronica Erma Barbisan

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldMedicine
TopicMonoclonal and Polyclonal Antibodies Research
Canadian institutionsToronto Metropolitan University
Fundersnot available
KeywordsReceptorCell biologyPhagocytosisPhospholipase CBiologyMolecular biologyChemistryBiochemistry

Abstract

fetched live from OpenAlex

The Fc receptor is expressed on the surface of RAW macrophages that can engulf IgG opsonized particles by phagocytosis. Isolation and characterization of these receptors and the identification of proteins involved is challenging. Live-cell Affinity Receptor Chromatography (LARC) (Janowski 2008) resulted in the identification of 336 proteins which are found to be specifically associated with the activated Fc receptor in RAW 264.7 macrophage cells. The proteins isolated were analyzed for protein-protein interactions using various analytical techniques including data mining through the use of iHOP, protein interaction databases such as Cytoscape, Osprey and STRING and literature searches, in order to create a signalling interaction pathway involving the proteins that are activated and associated with the Fcy receptor during receptor-mediated phagocytosis. The phospholipase C family of proteins have been previously identified as important in phagocytosis, and were identified by LARC. The selected isoforms were confirmed in RAW 264.7 cells via Western Blots, antibody staining, and live-cell confocal microscopy with fluorescently linked fusion proteins to verify the localization of the phospholipases, specifically PLC-β₃, PLC-β₄, PLC-y₁, PLC-d₁, PLC-ε₁ and PLC like -2 to the activated receptor complex.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.012

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.001
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0040.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.054
GPT teacher head0.358
Teacher spread0.304 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

Explore more

Same topicMonoclonal and Polyclonal Antibodies Research→French-language works237,207→