Tenacibaculum ovolyticum 16S rDNA Quantitative-PCR Assay Development and Field Testing
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
In British Columbia (BC; Canada) Atlantic salmon (Salmo salar L.) production, Tenacibaculum members are associated with ‘mouthrot’ and disease identification is based on gross observation and clinical data. Genomic similarities (i.e., putative virulence factors) between T. ovolyticum and other better-characterized agents of mouthrot could imply potential pathogenicity. While T. ovolyticum has not been directly linked to salmon mortality events in BC, it has been isolated from diseased marine fish. To investigate T. ovolyticum’s pathogenicity in situ, a T. ovolyticum 16S rDNA qPCR assay targeting a ~155 bp amplicon was developed. The assay was used to screen 67 biotic and 33 abiotic samples collected from a BC Atlantic salmon (Salmo salar L.) net-pen site before, during, and after a mouthrot outbreak. The assay was specific, quantifiable and detectable for T. ovolyticum over 6-log and 8-log units, respectively. However, cycle quotients differed between the BC isolate and type strain of T. ovolyticum, suggesting that qualitative use of the qPCR assay in field samples would be more accurate. Only two out of 100 samples were T. ovolyticum-positive, indicating limited involvement in this particular outbreak. However, the ecological role of T. ovolyticum and its involvement in the pathogenesis of other mouthrot outbreaks in Atlantic salmon is unknown.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it