Apoptosis-based topotecan-loaded superparamagnetic drug delivery system: an in vitro study
Bibliographic record
Abstract
Biological barriers could be overcome using nanobiotechnology, which promotes the development of nanomaterial-based delivery systems. The primary objective of the present investigation is superparamagnetic iron oxide nanoparticle (SPION) production for the delivery of topotecan to human breast cancer cells (MCF-7). The X-ray diffraction results confirmed the formation of pure SPIONs. The Fourier transform infrared spectra indicated the functional groups related to aminopropyl trimethoxy silane as a coating agent and topotecan. Topotecan-loaded magnetite nanoparticles with an IC 50 of approximately 156 μg/ml exhibited dose-dependent cytotoxicity. The polymerase chain reaction method also proved that in the mentioned cell line, topotecan-loaded SPIONs could increase the Bax/Bcl-2 ratio and p53 gene expression. An annexin V/propidium iodide detection assay was done to detect the induction of apoptosis. According to the results, the nanoparticles inhibit the survival of MCF-7 breast cancer cells by boosting apoptosis, which helps slow the growth of tumor cells.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".