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Record W4308514768 · doi:10.1016/j.celrep.2022.111624

HIV-1 Vpu restricts Fc-mediated effector functions in vivo

2022· article· en· W4308514768 on OpenAlexafffund
Jérémie Prévost, Sai Priya Anand, Jyothi Krishnaswamy Rajashekar, Li Zhu, Jonathan Richard, Guillaume Goyette, Halima Medjahed, Gabrielle Gendron‐Lepage, Hung-Ching Chen, Yaozong Chen, Joshua A. Horwitz, Michael W. Grunst, Susan Zolla‐Pazner, Barton F. Haynes, Dennis R. Burton, Richard A. Flavell, Frank Kirchhoff, Beatrice H. Hahn, Amos B. Smith, Marzena Pazgier, Michel C. Nussenzweig, Priti Kumar, Andrés Finzi

Bibliographic record

VenueCell Reports · 2022
Typearticle
Languageen
FieldImmunology and Microbiology
TopicHIV Research and Treatment
Canadian institutionsMcGill University Health CentreUniversité de MontréalMcGill UniversityCentre Hospitalier de l’Université de Montréal
FundersNational Institute of Diabetes and Digestive and Kidney DiseasesNational Institute of Allergy and Infectious DiseasesNational Heart, Lung, and Blood InstituteUniformed Services University of the Health SciencesNational Institutes of HealthUniversität zu KölnKarolinska InstitutetMinisterium für Wissenschaft, Forschung und Kunst Baden-WürttembergCanada Foundation for InnovationInstitute of Infection and ImmunityDeutsche ForschungsgemeinschaftNational Institute on Drug AbuseGruber FoundationVaccine Research CenterCanadian Institutes of Health ResearchBaden-Württemberg StiftungUniversity of MarylandFonds de Recherche du Québec - SantéTulane UniversityNational Institute of Neurological Disorders and StrokeNational Institute of General Medical SciencesInternational AIDS Vaccine Initiative
KeywordsAntibody-dependent cell-mediated cytotoxicityVirologyEffectorBiologyTetherinAntibodyEpitopeVirusGlycoproteinIn vivoCell biologyViral envelopeImmunologyMolecular biologyMonoclonal antibodyGenetics

Abstract

fetched live from OpenAlex

Non-neutralizing antibodies (nnAbs) can eliminate HIV-1-infected cells via antibody-dependent cellular cytotoxicity (ADCC) and were identified as a correlate of protection in the RV144 vaccine trial. Fc-mediated effector functions of nnAbs were recently shown to alter the course of HIV-1 infection in vivo using a vpu-defective virus. Since Vpu is known to downregulate cell-surface CD4, which triggers conformational changes in the viral envelope glycoprotein (Env), we ask whether the lack of Vpu expression was linked to the observed nnAbs activity. We find that restoring Vpu expression greatly reduces nnAb recognition of infected cells, rendering them resistant to ADCC. Moreover, administration of nnAbs in humanized mice reduces viral loads only in animals infected with a vpu-defective but not with a wild-type virus. CD4-mimetics administration, known to "open" Env and expose nnAb epitopes, renders wild-type viruses sensitive to nnAbs Fc-effector functions. This work highlights the importance of Vpu-mediated evasion of humoral responses.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesInsufficient payload (model declined to judge)
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.490
Threshold uncertainty score0.995

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0090.006

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.227
Teacher spread0.217 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; both teacher heads agree on what is shown here.

Study designNot applicable
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations30
Published2022
Admission routes2
Has abstractyes

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