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Record W4309070457 · doi:10.1371/journal.ppat.1010968

Differential immune transcriptomic profiles between vaccinated and resolved HCV reinfected subjects

2022· article· en· W4309070457 on OpenAlexafffund
Sabrina Mazouz, Eduardo Salinas, Nathalie Bédard, Ali Filali, Omar Khedr, Leo Swadling, Mohamed S. Abdel-Hakeem, Asiyah Siddique, Eleanor Barnes, Julie Bruneau, Arash Grakoui, Naglaa H. Shoukry

Bibliographic record

VenuePLoS Pathogens · 2022
Typearticle
Languageen
FieldMedicine
TopicHepatitis C virus research
Canadian institutionsUniversité de MontréalCentre Hospitalier de l’Université de Montréal
FundersOffice of Research Infrastructure Programs, National Institutes of HealthNational Center for Research ResourcesCanada Excellence Research Chairs, Government of CanadaNIHR Oxford Biomedical Research CentreFonds de Recherche du Québec - SantéNational Institute of Allergy and Infectious DiseasesMedical Research CouncilInstitute of Infection and ImmunityPublic Health AgencyCanadian Institutes of Health ResearchNational Institute for Health and Care ResearchNational Institutes of HealthPublic Health Agency of CanadaEmory UniversityStyrelsen för Internationellt Utvecklingssamarbete
KeywordsImmunologyVirologyImmune systemHepatitis C virusAntibodyTranscriptomeVaccinationMedicineBiologyVirusGeneGene expression

Abstract

fetched live from OpenAlex

Successive episodes of hepatitis C virus (HCV) infection represent a unique natural rechallenge experiment to define correlates of long-term protective immunity and inform vaccine development. We applied a systems immunology approach to characterize longitudinal changes in the peripheral blood transcriptomic signatures in eight subjects who spontaneously resolved two successive HCV infections. Furthermore, we compared these signatures with those induced by an HCV T cell-based vaccine regimen. We identified a plasma cell transcriptomic signature during early acute HCV reinfection. This signature was absent in primary infection and following HCV vaccine boost. Spontaneous resolution of HCV reinfection was associated with rapid expansion of glycoprotein E2-specifc memory B cells in three subjects and transient increase in E2-specific neutralizing antibodies in six subjects. Concurrently, there was an increase in the breadth and magnitude of HCV-specific T cells in 7 out of 8 subjects. These results suggest a cooperative role for both antibodies and T cells in clearance of HCV reinfection and support the development of next generation HCV vaccines targeting these two arms of the immune system.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.630
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.034
GPT teacher head0.274
Teacher spread0.240 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2022
Admission routes2
Has abstractyes

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