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Alarmin Expression in the Upper and Lower Airways of Asthmatics with Allergic Rhinitis

2022· article· en· W4312444528 on OpenAlexaff
Christiane E. Whetstone, Maral Ranjbar, Nadia Alsaji, Dhuha Al‐Sajee, Lesley Wiltshire, Jennifer Wattie, Paul M. O’Byrne, Ruth P. Cusack, Roma Sehmi, G.M. Gauvreau

Bibliographic record

Venuenot available
Typearticle
Languageen
FieldImmunology and Microbiology
TopicIL-33, ST2, and ILC Pathways
Canadian institutionsMcMaster University
Fundersnot available
KeywordsMedicineImmunologyAsthmaAllergic inflammationAllergic asthmaMucous membrane of noseAllergy

Abstract

fetched live from OpenAlex

<b>Rationale:</b> Allergic inflammation of the upper and lower airways may be manifestations of a single inflammatory process; hence, overexpression of epithelial alarmins should be present in both upper and lower allergic airways. To evaluate this we examined expression of TSLP and IL-33 in allergic asthmatics with comorbid allergic rhinitis. <b>Methods:</b> We collected endobronchial biopsies from allergic asthmatics (AA, n=23) non-asthmatic controls (NA, n=11), and inferior nasal turbinate biopsies from AA with comorbid allergic rhinitis (AR, n=8) and non-AA/non-AR controls (NR, n=5). Biopsies were stained for IL-33 and TSLP and immunopositive cells in epithelium and laminae propria were expressed per mm2 of tissue. <b>Results:</b> In the lower airways of AA there were more TSLP (p=0.021) and IL-33 (p=0.01) immunopositive cells than in the upper airways of AR. In controls TSLP (p=0.0018) but not IL-33 was more highly expressed in the lower airways than in upper airways.&nbsp;In lower airways IL-33 expression was greater in AA versus NA (p=0.005) with no difference in TSLP expression (p=0.31). In upper airways TSLP and IL-33 were expressed at similar levels in AR versus NR (p&gt;0.05). <b>Conclusion:</b> Both TSLP and IL-33 were more highly expressed in lower compared to upper airways of allergic subjects.&nbsp;This may reflect heightened sensitivity to stimuli detected in the lungs, even under baseline conditions. In the lower airways of allergic asthma IL-33 expression was higher than in controls.. However, TSLP is present at similar levels between allergic subjects and controls in both upper and lower airways demonstrating constitutive expression under baseline conditions.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.713
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.201
Teacher spread0.192 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2022
Admission routes1
Has abstractyes

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