Exogenous vitamin D inhibits human pro-inflammatory cytokine responses to TLR-4L but not TLR-8L innate immune activation (P6331)
Bibliographic record
Abstract
Abstract Population health studies demonstrate that insufficient/deficient Vitamin D status is strongly associated with increased upper respiratory tract infections. As low vitamin D status is widespread, it is imperative to understand the roles it plays during an immune response. Here we determine the effects of active vitamin D (1,25(OH)2D3) on peripheral blood mononuclear cells (PBMC) cytokine responses elicited by bacterial or viral ligands and explore mechanisms by which vitamin D regulates innate immune responses. Results: PBMC stimulated with TLR-4L and exogenous 1,25(OH)2D3 exhibited decreased pro-inflammatory cytokine production; whereas cultures stimulated with TLR-8L did not exhibit alterations in cytokine production patterns in the presence of supplemental 1,25(OH)2D3. 1,25(OH)2D3 alone slightly reduced TLR-4 but decreased TLR-8 by >75%. Moreover, while TLR-4 ligand stimulation had no effect on VDR levels, TLR-8L stimulation reduced VDR mRNA by 60% (p<0.005). Conclusions: Exogenous 1,25(OH)2D3 markedly reduces inflammatory cytokine production elicited by TLR-4 but not by TLR-8 or RSV activation. TLR-8L strikingly reduce VDR while 1,25(OH)2D3 reduces viral PRR expression, resulting in a synergistic decrease in the capacity of viral stimulated cells to have responses modulated by exogenous vitamin D. The results suggest a need for closer examination of the benefits of exogenous vitamin D supplementation in bacterial versus viral infection.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".