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Comparison of the TLR7 and TLR9 innate immune signaling pathways in african green monkeys and rhesus macaques

2016· article· en· W4313356436 on OpenAlexaff
I‐Ming Wang, Alexey Nefedov, Xiaohua Douglas Zhang, Sheri Dubey, Andrew Bett, Danilio Casimiro, Judith N. Mandl, Mark B. Feinberg

Bibliographic record

VenueThe Journal of Immunology · 2016
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmune Response and Inflammation
Canadian institutionsMcGill University
Fundersnot available
KeywordsTLR7Innate immune systemBiologyTLR9Immune systemImmunologyAcquired immune systemPattern recognition receptorPlasmacytoid dendritic cellDendritic cellToll-like receptorGeneGeneticsGene expression

Abstract

fetched live from OpenAlex

Abstract Innate immunity is an evolutionary mechanism which is involved in the initial detection of pathogens and stimulates the first line of host defense. The Toll-like receptor (TLR) family has been the most extensively studied innate immune pathway and it has been shown that TLRs can be activated in response to virtually any microbe which invades the host. These innate immune responses play a critical role in the development of pathogen-specific adaptive immune responses and disease pathology and are targeted by the use of adjuvants in modern vaccines. Importantly, divergent TLR7 and TLR9 signaling pathways between rhesus macaques and sooty mangabeys have been shown to distinguish between pathogenic and nonpathogenic AIDS virus infections in those species. Therefore, it is important to understand the TLR signaling pathways of non-human primate (NHP) species that are currently used for infectious disease and vaccine research. In the present study, we administered defined TLR7 and TLR9 agonists to two distinct NHP species, african green monkeys and rhesus macaques. We evaluated B cell, T cell, NK cell, monocyte, plasmacytoid dendritic cell and myeloid dendritic cell activation at 0, 24, 72 and 168 hours after dosing. Gene expression profiling and full pathway analysis was also evaluated to illuminate fundamental aspects of innate immune responses that underlie the mechanism of action of TLR-agonist adjuvants in these two NHP species.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.245
Teacher spread0.228 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2016
Admission routes1
Has abstractyes

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