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Bim is a candidate gene in the regulation of merocytic dendritic cell proportion

2016· article· en· W4313356463 on OpenAlexaff
Cindy Audiger, Geneviève Chabot‐Roy, Sylvie Lesage

Bibliographic record

VenueThe Journal of Immunology · 2016
Typearticle
Languageen
FieldImmunology and Microbiology
TopicPhagocytosis and Immune Regulation
Canadian institutionsUniversité de MontréalHôpital Maisonneuve-Rosemont
Fundersnot available
KeywordsCongenicBiologyAutoimmunityChimera (genetics)Locus (genetics)GeneNodT cellImmunologyCell biologyGeneticsImmune system

Abstract

fetched live from OpenAlex

Abstract In contrast to conventional dendritic cells (cDC), when merocytic DC (mcDC) present antigens (Ag) derived from apoptotic bodies, T cell anergy is reversed rather than induced. Although helpful to tumour clearance, reversing T cell anergy is detrimental in autoimmunity. Interestingly, mcDC are present in higher proportion in type 1 diabetes (T1D)-prone NOD mice than in B6 mice. Recently, we found that the Idd13 T1D locus is linked to the control of mcDC number. Indeed, NOD.B6-Idd13 congenic mice are resistant to T1D and have the same low number of mcDC as the parental B6 mice. Thus, we hypothesize that defining the genetic factor implicated in the regulation of mcDC number could help maintain self-tolerance and prevent T1D onset. Within the Idd13 locus, both B2m and Bim are likely candidate genes. By exploiting both B2m−/−and Bim−/−mice, we show that B2m does not affect mcDC number while mcDC were significantly increased in Bim−/−mice. Competitive hematopoietic chimera validate Bim as an intrinsic factor implicated in the regulation of mcDC proportion. In line with a role for Bim in regulating mcDC number, we demonstrate that the caspase activity and Bcl2 expression is not differentially expressed between the different mouse model, suggesting that Bim is implicated in mcDC proportion regulation via a caspase-independent pathway. Together, these data demonstrate that Bim, encoded within the Idd13 locus on the chromosome 2 modulates the number of mcDC. Identifying factors that facilitate apoptosis of mcDC may help prevent autoimmunity. Still, whether restoring Bim expression in NOD mice would prevent T1D remains to be addressed.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.230
Teacher spread0.218 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2016
Admission routes1
Has abstractyes

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