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Staphylococcal superantigens trigger rapid human IL-17A production by a novel population of memory T cells (HUM1P.268)

2015· article· en· W4313356676 on OpenAlexaff
Peter A. Szabo, Ankur Goswami, John K. McCormick, S. M. Mansour Haeryfar

Bibliographic record

VenueThe Journal of Immunology · 2015
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmune Response and Inflammation
Canadian institutionsWestern University
Fundersnot available
KeywordsSuperantigenToxic shock syndromePeripheral blood mononuclear cellImmunologyCytokineStaphylococcus aureusPathogenesisBiologyPopulationEnterotoxinInflammationSystemic inflammatory response syndromeIn vitroMicrobiologyMedicineSepsisT cellImmune systemBacteriaGeneEscherichia coli

Abstract

fetched live from OpenAlex

Abstract Toxic shock syndrome (TSS) is a life-threatening illness characterized by fever, hypotensive shock and multi-organ failure within hours of onset. TSS is caused by exposure to bacterial toxins known as superantigens (SAgs), which are produced by common Gram-positive bacteria such as Staphylococcus aureus. During infection, SAgs can activate of up to 20% of all T cells, resulting in massive and rapid release of pro-inflammatory cytokines, systemic intravascular coagulation, organ damage and death. However, the exact cellular mechanisms underlying the rapidity of this potentially fatal inflammatory reaction are far from clearly understood. In our preliminary experiments, human peripheral blood mononuclear cells (PBMCs) stimulated with SAgs in vitro showed immediate up-regulation (up to 2000-fold) of mRNA for the pro-inflammatory cytokine IL-17A. SAg-stimulated PBMCs also produced substantial amounts of IL-17A protein within hours of activation. The cells responsible for the rapid production of IL-17A were identified as a subset of memory T cells exhibiting a novel phenotype. Furthermore, neutralizing IL-17A at the onset significantly reduced both morbidity and mortality in a humanized mouse model of TSS. Our results reveal a critical role for memory T cells in TSS and define a novel contribution by IL-17A to the initiation and pathogenesis of the syndrome.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.240
Teacher spread0.221 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2015
Admission routes1
Has abstractyes

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