The ion channel TRPM7 is required for B cell lymphopoiesis
Bibliographic record
Abstract
Abstract The ion channel, transient receptor potential subfamily melastatin, member 7 (TRPM7) was first identified as critical for cell viability in the B cell line, DT40, by regulating cellular Mg2+ homeostasis. However, specific deletion of TRPM7 in T cells results in only a minor defect in thymocyte development and nearly normal numbers of peripheral T cells, raising the question of whether TRPM7 is in fact important for B cell development in vivo. Here, we generated a murine model in which TRPM7 is specifically deleted in B cells under control of the Mb1 promotor. We show that Mb1-Cre mediated deletion of TRPM7 results in a complete loss of peripheral B cells and a developmental block at the pro B cell stage in the bone marrow, coincident with the expression of Mb1. Importantly, we demonstrate using the in vitro OP9 stromal cell system of lymphopoiesis that supplementation with high levels of extracellular Mg2+ can partially support B cell development in TRPM7-deficient mice. Thus, our findings identify a critical role for TRPM7 in B cell lymphopoiesis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".