Endothelial-expressed leukocyte-specific protein 1 regulates intravascular and extravascular directionality of chemotaxing neutrophils (P5094)
Bibliographic record
Abstract
Abstract The endothelial-expressed Ca2+- and F-actin-binding leukocyte-specific protein 1 (LSP1) was shown to play a pivotal role in the regulation of endothelial transmigratory cup formation, microvascular permeability increases, and transendothelial migration of leukocytes. Due to the complexity of leukocyte-endothelial interactions, the cell-specific role of LSP1 is incompletely understood. To elucidate the orchestration of neutrophil dynamics by endothelial LSP1 in vivo, intravital microscopy and time-lapsed video analysis were performed in the microvasculature of cremaster muscle in LSP1-deficient chimeric mice. Absence of LSP1 (Lsp1−/−) in non-hematopoietic tissue resulted in significantly impaired chemotactic directionality (chemotaxis index) but not the neutrophil migration velocity during CXCL2-elicited intraluminal crawling and extravascular migration as compared to the responses in wild type (Lsp1+/+) endothelial cells. The velocity of migration of Lsp1−/− neutrophils in extravascular tissue was significantly lower in both Lsp1+/+ and Lsp1−/− chimeric mice as compared to that of Lsp1+/+ neutrophils in these mice. Analysis of integrin expression of transmigrated neutrophils from CXCL2-induced peritonitis in chimeric mice revealed blunted expression of α6 and β1 on neutrophils only in mice with Lsp1−/− endothelial cells. In conclusion, non-hematopoietic endothelial LSP1 is a novel regulator of neutrophil directionality during neutrophil recrutment.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".