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Record W4313521904 · doi:10.21203/rs.3.rs-2381206/v1

M2 macrophages are the origin of tumor metastasis

2023· preprint· en· W4313521904 on OpenAlexaff
Hui Li, Yingqi Miao, Liping Suo, Xi Wang, Yiqing Mao, Xuehui Zhang, Na Zhou, Junrui Tian, Xiuyan Yu, Tongxia Wang, Yan Gao, Hongyan Guo, Zheng Zhang, Diansheng Ma, Hongxia Wu, Yanwei Cui, Xiliang Zhang, Xiaochun Chi, Yu‐Chi Li, David M. Irwin, Gang Niu, Huanran Tan

Bibliographic record

VenueResearch Square · 2023
Typepreprint
Languageen
FieldImmunology and Microbiology
TopicPhagocytosis and Immune Regulation
Canadian institutionsUniversity of Toronto
Fundersnot available
KeywordsMetastasisCancer researchIn vivoCancer cellOvarian cancerPhagocytosisCancerMacrophageGene knockdownMedicineBiologyCell cultureIn vitroImmunologyInternal medicine

Abstract

fetched live from OpenAlex

Abstract Tumor metastasis is a key factor affecting the life of patients with malignant tumors. For the past hundred years, scientists have been focusing on how to kill cancer cells and inhibit their metastasis in vivo, but few breakthroughs have been made. Here we propose a novel mode for cancer metastasis. Here we show that the phagocytosis of apoptotic tumor cells by macrophages leads to their polarization into the M2 phenotype, and that the expression of stem cell related as well as drug resistance genes are induced. Therefore, it appears that M2 macrophages have "defected" and have been transformed into the initial "metastatic cancer cells", and thus are the source of the distal tissue tumor metastasis. This conclusion is supported by the presence of fused cells with both macrophage and tumor cell characteristics in the peripheral blood and ascites of patients with ovarian cancer. By suppressing the expression of CD206 in M2 macrophages by siRNA, we show that the growth and metastasis of tumors is suppressed at the in vitro cell line and in the in vivo experimental mice models. In summary, we show that M2 macrophages in the blood circulation undergo a "change of loyalty" to become "cancer cells" that undergo distal tissue metastasis, which can be suppressed by the knockdown of CD206 expression.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.130
GPT teacher head0.404
Teacher spread0.273 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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