Analysis on SSR Loci in Transcriptome and Development of EST-SSR Molecular Markers in <i>Lonicera macranthoides</i>
Bibliographic record
Abstract
Huizhanmao-rendong ( Lonicera macranthoides ), as a species of original plant of wild honeysuckle flower ( Flos Lonicerae ), the traditional Chinese medicine, is widely distributed in South China and features high medicinal and economic value. In this study, the SSR loci of 74 057 unigenes in the transcriptome of L . macranthoides were analyzed by MISA. Among them, 15 587 unigenes contained 20 161 SSR loci, and the frequency of SSR was 21.05%. The types of SSR sites in the transcriptome of L . macranthoides were distributed from single nucleotide to six nucleotide repeats. The main repeats were dinucleotide (9 704) and trinucleotide (5 847), accounting for 48.13 % and 29.00 % of the total SSRs, respectively. The main repeat units were AG/CT (6 086), AT/AT (3 071) and A/T (2 826), accounting for 30.79%, 15.23%, and 14.02% of the total SSRs, respectively. There were 4 897 and 3 531 repeats (the numbers of repeats were the most) in 6 and 5 SSR loci, accounting for 24.29 % and 17.51 % of the total SSR number, respectively. By using primer 6.0 software, 17 611 pairs of SSR primers were designed, and 30 pairs of primers were randomly selected for amplification verification. Among them, 14 pairs of amplified bands showed polymorphism, and then the genetic diversity of 6 varieties of L . macranthoides was analyzed by using these 14 pairs of primers. The results will provide a scientific basis for species identification, genetic diversity analysis, and molecular marker-assisted breeding of L . macranthoides .
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".