Cloning and Expression Analysis of MaMYB308 Gene in Mulberry
Bibliographic record
Abstract
In order to analyze the function of MYB transcription factors in mulberry abiotic stress and development, the full-length cDNA sequence of MYB transcription factor MaMYB308 was cloned from the mulberry cultivar ‘Guiyou 62’. The amino acid sequence of MYB transcription factor MaMYB308 was analyzed by bioinformatics, and its self activation was verified by yeast single hybridization. The expression pattern of the gene under different tissue and abiotic stresses was detected by real-time fluorescence quantitative PCR. The results showed that the full length of MaMYB308 gene is 1 266 bp, including an open reading frame of 1 026 bp, which can encode 341 amino acids, and its molecular weight is 39 kD; it has two SANT domains, belonging to typical R2R3 type MYB transcription factor, and has self activating activity. Blast results showed that MaMYB308 had high homology with a variety of R2R3-MYB transcription factors from higher plants, and had the highest homology with MYB308 from Morus notabilis , and the consistency of amino acid level was 96.48%. The results of real-time fluorescence quantitative PCR showed that the expression of MaMYB308 in roots, stems and leaves of mulberry was significantly higher than that in stems and leaves, and it was responsive to abiotic stresses such as high temperature, low temperature, high salt and drought. Among them, the expression of MaMYB308 was the most significant under low temperature stress, about 40 times of the control. The results of this study could be helpful for further study on the biological function of mulberry MaMYB308 gene and the stress resistance mechanism of mulberry.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".