Characteristics of <i>LDOX</i> Gene Structure Analysis and Construction of Protein Interaction Network in <i>Actinidia arguta</i>
Bibliographic record
Abstract
The purpose of this study is to reveal the structure and function of the leucoanthocyanidin dioxygenase ( LDOX ) gene in Actinidia arguta . Bioinformatics methods were used to analyze the LDOX nucleotide and amino acid sequences of Actinidia arguta , construct the protein interaction network of LDOX gene, and conduct GO and KEGG functional annotation analysis. The results showed that there were 10 conserved motifs in Actinidia arguta , which was closely related to Vaccinium corymbosum , Lonicera japonica and Daucus carota . Cis-regulated elements were mainly involved in ABA signaling pathways, responding to drought response, anaerobic induction, gibberellic acid, auxin response factor, MeJA response, low temperature response, and photostimulus response. Physical and chemical properties and structural analysis of LDOX showed that it was hydrophilic protein, and no signal peptide and transmembrane structural regions were found, mainly including Ser phosphorylation sites (13), Thr phosphorylation sites (12) and Tyr phosphorylation sites (6). There were two O-glycosylation sites (Thr 4 and Thr 323 ) and Asn 107 of N- glycosylation sites at residues ranging from 107 to 109. There was a domain structure conservative in 48-347 bp was 20G-FeⅡ Oxy. The secondary structure main random coil (43.10%). There was a certain interaction between LDOX and proteins such as F3H, UFGT, FLS and PAL, which involved in flavonoid biosynthesis and metabolism process that plays an important role in plant-type vacuole membrane, and regulates the function of catalytic activity and redox active molecule. LDOX gene was highly conserved and plays an important regulatory role in progesterone pathway, which provides a theoretical basis for further research on the functional mechanism of LDOX gene in Actinidia arguta .
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".