Dynamic remodelling of cadherin contacts in embryonic mesenchymal cells during differential cell migration
Bibliographic record
Abstract
Abstract A fundamental aspect of morphogenesis is the capacity of cells to actively exchange neighbours while maintaining the overall cohesion of the tissue. These cell rearrangements require the dynamic remodelling of cadherin cell adhesions. Many studies have examined this process in tissues where it is driven by the joint action of cell protrusions and actomyosin contraction along the shrinking junction. However, cell rearrangements can also occur through differential migration. This mode of cell rearrangement, characteristic of mesenchymal tissues, is much less well understood. Here, we explore the prototypical case of the gastrulating Xenopus prechordal mesoderm, and provide the first detailed analysis at how cadherin contacts are remodelled and eventually disrupted in this type of tissue. Using a reductionist approach, including analysis of single contacts using a dual pipette aspiration setup, we unveil two concurrent mechanisms. Most cadherins are removed via “peeling”, i.e. disruption of the trans bonds and lateral diffusion out of the contact. In parallel, a remnant of cadherins concentrates at the shrinking contact, which is ultimately resolved by breakage of the link with the actin cytoskeleton, showing that the weakest link shifts at different stages of contact remodelling. Additionally, we observe recruitment of myosin peripheral to the shrinking contact, which influences the efficiency of the final detachment. Finally, manipulation of cortical tension indicates that the process is sensitive to the magnitude and orientation of the forces applied on the contact, revealing another key relationship between cell-cell adhesion and the cortical cytoskeleton. This study unravels a new modality of cell contact dynamics, which is likely to be widely relevant for highly migratory mesenchymal tissues.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".