Zoospore production and motility of mangrove thraustochytrids from Hong Kong under various salinities
Bibliographic record
Abstract
We investigated the effects of salinity on the zoospore production of four mangrove thraustochytrid isolates, Schizochytrium sp. KF1, Aurantiochytrium mangrovei KF6, Thraustochytrium striatum KF9 and Ulkenia sp. KF13. The zoospore motilities, which were based on curvilinear velocity (VCL) and straight-line velocity (VSL), were monitored using the Computer-Assisted Sperm Motility Analysis (CASA) Software system. The zoospore production of four isolates was suppressed at salinity above 15%. Schizochytrium sp. produced the greatest number of zoospores at 15%, while Aurantiochytrium mangrovei and Ulkenia sp. produced abundant zoospores in diluted sea water ranging from 7.5 to 15%. Thraustochytrium striatum performed relatively poorly under all salinities. Salinity and exposure time, as well as their interactions, had significant impacts on most zoospore velocity measurements. The optimal velocities of zoospore motility also varied among isolates. Zoospores of Schizochytrium sp. and A. mangrovei had similar responses to salinity, with the highest motility at 7.3%, followed by a decrease in velocities with increasing salinity. In contrast, the zoospore of T. striatum had optimal motility at 12% and remained highly motile from 15 to 20%. The velocities of zoospores of Ulkenia sp. were the lowest among the tested thraustochytrids and had optimal motility at 12%. Zoospores of all the isolates remained active after 4 h of exposure to aqueous medium, but the optimal salinity for each mode of swimming changed. The ecological significance of these data are discussed.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".