Cloning and Characterization of Aquaporins 11 and Its Expression Analysis under Molt Cycle in <i>Eriocheir sinensis</i>
Bibliographic record
Abstract
Aquaporins play important roles in water molecule transport, ion transport, and osmotic pressure regulation. In this study, the full-length cDNA of aquaporin from Chinese mitten crab ( Eirocheir sinensis ) was cloned for first time using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends analyses. The full-length of AQP11 cDNA is 1746bp, with a 463bp 5’-untranslated region (UTR), a 476bp 3’-UTR, and an open reading frame (ORF) of 807 bp which encodes a 269 amino acid polypeptide. The molecular mass of the predicted protein is 29.46 kDa with an estimated PI of 5.38. Bioinformatics software analysis revealed that AQP11 gene contains 4 transmembrane domains, 2 NPV structural units. Homologous analysis showed that AQP11 of Eirocheir sinensis has the highest homology to AQP11 of Litopenaeus vanname i . Real-time quantitative RT-PCR showed that AQP11 gene could be detected in all tested tissues of Eirocheir sinensis , with the highest expression level in intestine, followed by the brain, muscle and thoracic ganglion, and the lowest expression level in the hepatopancreas, gills and blood. In the intestinal, the expression of AQP11 was significantly lower at the intermolt stage (stages C) and premolt stage (stage D), significantly enhanced and reached the maximal level at the ecdysis stage (stage E). On the other hand, the expression of AQP11 gene in muscle showed low expression in the intermolt stage (stage C), and it was increased gradually at the premolt stage (stage D), significantly enhanced and reached the maximal level at the ecdysis stage (E stage), and then reaching the postmolt stage(AB stage) decline. In summary, our results indicate that AQP11 may play an important role in molting of Enirocheir sinensis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".