Poster Presentations
Bibliographic record
Abstract
Problem: Epidemiological studies in humans implicate maternal expression of different combinations of KIRs, which are a family of cell surface receptors that tune cellular activation status, as being predictive in pregnancy complication onset (e.g., preeclampsia) and fetal health.This receptor family is expressed in both innate and adaptive immune cell populations, including the most abundant cell types found in the pregnant uterus.Due to limitations in studying human samples, a mouse model was generated in which the functionally homologous receptor family, Ly49s, has been globally deleted.The deletion of this gene complex leads to impaired vasculature formation and remodeling in mid-pregnancy, ultimately driving pregnancy defects, mirroring the correlations seen in the human population.However, the problem is that the mechanisms by which the lack of Ly49 receptors change immune cell function and leads to deleterious pregnancy outcomes remains unknown. Method of Study:To investigate the contributions of Ly49 receptors in pregnancy associated angiogenesis, we mated wildtype (WT) and Ly49-/-dams with WT sires and analyzed the implantation sites at gestational day 9.5.We performed a proteomic screen for expression of proteins known to be involved in angiogenesis, followed by verification via flow cytometry and western blot.Additionally, cultured WT and Ly49-/-uterine single cell suspensions were analyzed by flow cytometry and conditioned media was utilized an in-vitro angiogenesis assay using a murine endothelial cell line, SVEC4-10, on a Geltrex extracellular matrix bed.Endothelial tube formation was imaged by light microscopy and quantified using ImageJ.Results: Ly49-/-dams at gestational day 9.5 display a skewed expression of angiogenesis related proteins, as compared to WT dams, with several proteins having significantly decreased abundance.VEGF expression in particular is 2-3-fold lower in Ly49-/-implantation sites, with this decrease coming from a non-immune cell population.Cultured Ly49-/-uterine cells display an increased production of pro-inflammatory cytokines compared to WT, including TNFa and IFNg, whose cellular source is from a monocyte/macrophage lineage.Conditioned media from Ly49-/-uterine cells leads to a significant anti-angiogenic effect, with a substantial decrease in endothelial tube branching and pronounced morphological changes. Conclusion:Together, these data indicate that loss of Ly49 receptors is leading to dysregulated immune cell function and production of
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".