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Record W4376134693 · doi:10.1038/s41593-023-01326-3

ARG1-expressing microglia show a distinct molecular signature and modulate postnatal development and function of the mouse brain

2023· article· en· W4376134693 on OpenAlexafffund
Vassilis Stratoulias, Rocío Ruiz, Shigeaki Kanatani, Ahmed M. Osman, Lily Keane, José Á. Armengol, Antonio Rodríguez Moreno, Adriana‐Natalia Murgoci, Irene García-Domínguez, Isabel M. Alonso-Bellido, Fernando Gonzàlez Ibáñez, Katherine Picard, Guillermo Vázquez-Cabrera, Mercedes Posada-Pérez, Nathalie Vernoux, Darío Tejera, Kathleen Grabert, Mathilde Cheray, Patricia González-Rodríguez, Eva M. Pérez-Villegas, Irene Martínez‐Gallego, Alejandro Lastra Romero, David Brodin, Javier Avila‐Cariño, Yang Cao, Mikko Airavaara, Per Uhlén, Michael T. Heneka, Marie‐Ève Tremblay, Klas Blomgren, José L. Venero, Bertrand Joseph

Bibliographic record

VenueNature Neuroscience · 2023
Typearticle
Languageen
FieldNeuroscience
TopicNeuroinflammation and Neurodegeneration Mechanisms
Canadian institutionsUniversité LavalUniversity of Victoria
FundersJunta de AndalucíaBarncancerfondenSigrid Juséliuksen SäätiöMinisterio de Ciencia e InnovaciónUniversity of VictoriaVetenskapsrådetHjärnfondenRadiumhemmets ForskningsfonderCancerfondenKarolinska InstitutetEuropean CommissionSvenska KulturfondenAcademy of FinlandSwedish Cancer FoundationÅke Wiberg StiftelseFonds de Recherche du Québec - SantéWenner-Gren Foundation
KeywordsMicrogliaNeuroscienceBiologyHippocampusForebrainBasal forebrainCholinergicArginaseLong-term potentiationCholinergic neuronCentral nervous systemImmunologyInflammationReceptorGenetics

Abstract

fetched live from OpenAlex

Abstract Molecular diversity of microglia, the resident immune cells in the CNS, is reported. Whether microglial subsets characterized by the expression of specific proteins constitute subtypes with distinct functions has not been fully elucidated. Here we describe a microglial subtype expressing the enzyme arginase-1 (ARG1; that is, ARG1 + microglia) that is found predominantly in the basal forebrain and ventral striatum during early postnatal mouse development. ARG1 + microglia are enriched in phagocytic inclusions and exhibit a distinct molecular signature, including upregulation of genes such as Apoe , Clec7a , Igf1 , Lgals3 and Mgl2 , compared to ARG1 – microglia. Microglial-specific knockdown of Arg1 results in deficient cholinergic innervation and impaired dendritic spine maturation in the hippocampus where cholinergic neurons project, which in turn results in impaired long-term potentiation and cognitive behavioral deficiencies in female mice. Our results expand on microglia diversity and provide insights into microglia subtype-specific functions.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.013
Threshold uncertainty score0.613

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.240
Teacher spread0.225 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations94
Published2023
Admission routes2
Has abstractyes

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