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Record W4378903416 · doi:10.1038/s41598-023-35723-2

Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research

2023· article· en· W4378903416 on OpenAlexaff
Jane Shaw, Maynard Meiring, Devon Allies, Lauren Cruywagen, Tarryn-Lee Fisher, Kesheera Kasavan, Kelly Roos, Stefan Botha, Candice MacDonald, Andriёtte M. Hiemstra, Donald Simon, Ilana van Rensburg, Marika Flinn, Ayanda Shabangu, Helena Kuivaniemi, Gerard Tromp, Stephanus T. Malherbe, Gerhard Walzl, Nelita du Plessis, Elisa Nemes, Léanie Kleynhans, Shirley McAnda, Charlene Kruger, Tracey Richardson, Firdows Noor, Lauren Benting, Nosipho Mtala, Robin-Lee Storm, Gertrude Persence, Elsabe Molima, Alicia Chetram, Kim Stanley, David Lewinsohn, Deborah A. Lewinsohn, Kevin B. Urdahl, Erwin Schurr, Marianna Orlova, W. Henry Boom, Sarah M. Fortune

Bibliographic record

VenueScientific Reports · 2023
Typearticle
Languageen
FieldMedicine
TopicInhalation and Respiratory Drug Delivery
Canadian institutionsMcGill University Health Centre
FundersEuropean and Developing Countries Clinical Trials PartnershipFaculty of Medicine and Health, University of SydneyDivision of Research Capacity DevelopmentFaculty of Medical and Health Sciences, University of AucklandNational Institutes of HealthUniversiteit StellenboschMedical Research CouncilSouth African Medical Research CouncilNational Research FoundationNational Institute of Allergy and Infectious DiseasesEuropean Commission
KeywordsBronchoalveolar lavageMedicineImmunologyTuberculosisImmune systemDiseaseInfectious disease (medical specialty)Internal medicineLungPathology

Abstract

fetched live from OpenAlex

Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and cell yield, concentration, viability, pellet colour and differential count were analysed for association with important participant characteristics such as active tuberculosis (TB) disease, TB exposure, HIV infection and recent SARS-CoV-2 infection. In 337 participants, BAL volume and BAL cell count were correlated in those with active TB disease, and current smokers. The right middle lobe yielded the highest volume. BAL cell and volume yields were lower in older participants, who also had more neutrophils. Current smokers yielded lower volumes and higher numbers of all cell types, and usually had a black pellet. Active TB disease was associated with higher cell yields, but this declined at the end of treatment. HIV infection was associated with more bloody pellets, and recent SARS-CoV-2 infection with a higher proportion of lymphocytes. These results allow researchers to optimise their participant and end assay selection for projects involving lung immune cells.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.019
metaresearch head score (Gemma)0.017
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.019
Threshold uncertainty score0.098

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0190.017
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0010.001
Scholarly communication0.0020.001
Open science0.0010.002
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0060.004

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.158
GPT teacher head0.403
Teacher spread0.245 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations10
Published2023
Admission routes1
Has abstractyes

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