Identification and Expression Profiles of the WRKY Gene Family in Pecan (<i>Carya illinoinensis</i>)
Bibliographic record
Abstract
WRKY gene family encodes a large transcription factor family that play critical roles in various physiological processes. However, a systematic analysis of the WRKY transcription factor family has not been reported in pecan ( Carya illinoinensis ). In this study, a total of 89 putative pecan WRKY genes ( CiWRKYs ), named CiWRKY1-89 , were identified from the whole genome of pecan. Most of WRKY domain sequences in CiWRKY proteins were “WRKYGQK”, but there were “WRKYGKK” in CiWRKY19, CiWRKY27 and CiWRKY88, and “WKKYGQK” in CiWRKY85. All CiWRKYs were unevenly distributed on 16 chromosomes, the largest number of genes on chromosome 3. Based on phylogenetic analysis, the 89 putative CiWRKYs could be classified into three major groups. The CiWRKY genes shared similar exon-intron distribution, and conserved motifs within the same subgroups. Expression profiles indicated that CiWRKY16 , CiWRKY30 , CiWRKY42 , CiWRKY62 , and CiWRKY69 genes were involved in flower differentiation and development, and majority of CiWRKYs genes were differentially expressed during embryo development. The present study provides reference for further comparative genomics and functional studies of this important class of transcriptional regulators in pecan.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".