Abstract 15338: The Essential Role of Fatty-Acid Binding Protein 3 in Endothelial Function
Bibliographic record
Abstract
Background: The endothelium lines blood vessel’s inner walls and interacts directly with the circulation, systemically influencing whole-body physiology through endothelial function. Fatty acid binding protein 3 (FABP3), abundant in myocardiocytes, serve as effective cardiac injury’s biomarkers when presented in circulation. Our lab has preliminary found that endothelial cells (ECs) express FABP3. The role of FABP3 in endothelial function and FABP3 exposure’s effects on ECs are unknown. Objective: Evaluate FABP3’s role in endothelial dysfunction. Method: Human umbilical vein endothelial cells (HUVECs) were grown under cell culture standards (370 C, 5% CO2) prior to treatment by lipopolysaccharide (LPS). FABP3 expression in treated HUVECs was evaluated by qPCR. Next, FABP3-silencing RNAs were transfected into HUVECs to knock-down FABP3 for 24 hours. HUVECs were also exposed to recombinant FABP3 as a gain of function model. HUVECs were then treated with LPS for 24 hours before subjected to molecular/phenotypic evaluation for aspects of endothelial function (migration, proliferation, angiogenesis, inflammation). The Delta-Ct method and One-way ANOVA with Tukey (HSD) test were used to analyze data from qPCR and comparisons between treatment groups, respectively; p-value < 0.05 is considered significant. Results: 1) FABP3 is basally expressed in ECs, 2) LPS upregulated endothelial FABP3 expression; 3) loss of FABP3 protected ECs against LPS-induced endothelial dysfunction; FABP3 exposure exacerbated LPS-induced inflammation; 4) loss of FABP3 protects ECs against LPS-induced stress by promoting cell survival, anti-inflammatory and pro-angiogenic pathways. Conclusions: We identified the novel and basal expression of FABP3 in ECs and demonstrated that FABP3 influences endothelial function at both the molecular and phenotypic levels under stress in vitro. Loss- and gain- of endothelial FABP3 ameliorates and exacerbates LPS-induced endothelial dysfunction, respectively, by affecting cell survival and inflammatory and angiogenic pathways. Our findings suggest that circulating FABP3 may be detrimental to the endothelium; therefore, therapies aimed at inhibiting FABP3 may improve endothelial function in myocardial injury.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.011 | 0.004 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".