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Abstract 11904: Lipoprotein(a) Integrates Monocyte-Mediated Thrombosis and Inflammation in Atherosclerotic Cardiovascular Disease

2022· article· en· W4380794364 on OpenAlexaff
Robert S. Rosenson, Ashley Tate, Qinzhong Chen, Olga Grushko, Dilna Damodaran, Priscilla Mejia, Michael B. Boffa, Marlys L. Koschinsky, Jagat Narula, Sascha N. Goonewardena

Bibliographic record

VenueCirculation · 2022
Typearticle
Languageen
FieldMedicine
TopicLipoproteins and Cardiovascular Health
Canadian institutionsWestern University
Fundersnot available
KeywordsInflammationMonocyteMedicineTissue factorLipoprotein(a)CD14ImmunologyInternal medicineLipoproteinThrombosisReceptorCholesterolCoagulation

Abstract

fetched live from OpenAlex

Introduction: Lipoprotein (a) [Lp(a)] drives atherosclerotic cardiovascular disease (ASCVD) through multiple mechanisms including cholesterol accumulation, inflammation, and thrombosis. However, the effects of Lp(a) on monocyte activation and monocyte-mediated thrombosis are unknown. Hypothesis: Lp(a) itself can activate monocytes, driving inflammation and thrombosis in parallel. Methods: We employed systems biology approaches consisting of proteomics, transcriptomics, and mass cytometry to define the immune cellular and molecular phenotypes in ASCVD subjects with high and low Lp(a) levels and leveraged cell models to define the mechanisms through which Lp(a) drives monocyte inflammation and thrombosis. We included study participants with CHD who were aged 18 to 80 years with Lp(a) concentrations > 150 nmol/L (cases) and <75 nmol/L (controls). Results: Circulating markers of inflammation (CCL3, CD40) and vascular dysfunction (PAR1, tissue factor [TF]) were elevated in subjects with high Lp(a) levels compared with those with low Lp(a) levels. Additionally, although total monocytes levels and hs-CRP levels were similar between the groups, CD14+ monocytes from ASCVD subjects with an elevated Lp(a) were primed and expressed more TF at baseline and in response to stress. Mechanistically, we found that Lp(a) itself can activate monocytes through Toll-like receptors (TLR) and nuclear factor kappa B (NFκB) signaling, driving both TF expression and TF activity (Figure). Conclusions: Overall, these studies are the first to link Lp(a) to monocyte-mediated inflammation and thrombosis in ASCVD subjects, demonstrating a novel mechanism through TLR2, NFκB, and monocyte TF through which Lp(a) amplifies immunothrombotic risk.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0050.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.032
GPT teacher head0.257
Teacher spread0.225 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2022
Admission routes1
Has abstractyes

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