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Record W4382138646 · doi:10.1038/s41467-023-39487-1

NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice

2023· article· en· W4382138646 on OpenAlexafffund
Le Son Tran, Le Ying, Kimberley D’Costa, Georgie Wray‐McCann, Genevieve Kerr, L. Le, Cody C. Allison, Jonathan Ferrand, Hassan Chaudhry, Jack Emery, Amanda De Paoli, Nina Colon, Sarah J. Creed, Maria Kaparakis‐Liaskos, Julia Como, Jennifer K. Dowling, Priscilla Johanesen, Thomas A. Kufer, Jakob Skou Pedersen, Ashley Mansell, Dana J. Philpott, Kirstin Elgass, Helen E. Abud, Ueli Nachbur, Ben A. Croker, Seth L. Masters, Richard L. Ferrero

Bibliographic record

VenueNature Communications · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicInflammasome and immune disorders
Canadian institutionsUniversity of Toronto
FundersMonash Biomedicine Discovery Institute, Monash UniversityNational Health and Medical Research CouncilHudson Institute of Medical ResearchState Government of VictoriaUniversity of MelbourneMonash UniversityMedical Research CouncilDeutscher Akademischer AustauschdienstPublic Health AgencyPublic Health Agency of CanadaU.S. Department of Defense
KeywordsInflammasomeNOD1Cell biologyHelicobacter pyloriImmunologyBiologyInterleukinPyrin domainMyeloidInnate immune systemInflammationCytokineImmune systemNOD2Genetics

Abstract

fetched live from OpenAlex

The interleukin-1 family members, IL-1β and IL-18, are processed into their biologically active forms by multi-protein complexes, known as inflammasomes. Although the inflammasome pathways that mediate IL-1β processing in myeloid cells have been defined, those involved in IL-18 processing, particularly in non-myeloid cells, are still not well understood. Here we report that the host defence molecule NOD1 regulates IL-18 processing in mouse epithelial cells in response to the mucosal pathogen, Helicobacter pylori. Specifically, NOD1 in epithelial cells mediates IL-18 processing and maturation via interactions with caspase-1, instead of the canonical inflammasome pathway involving RIPK2, NF-κB, NLRP3 and ASC. NOD1 activation and IL-18 then help maintain epithelial homoeostasis to mediate protection against pre-neoplastic changes induced by gastric H. pylori infection in vivo. Our findings thus demonstrate a function for NOD1 in epithelial cell production of bioactive IL-18 and protection against H. pylori-induced pathology.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0000.000
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.301
Teacher spread0.288 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations23
Published2023
Admission routes2
Has abstractyes

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Same venueNature CommunicationsSame topicInflammasome and immune disordersFrench-language works237,207