Optimization of ISSR-PCR Reaction System in <i>Pinellia ternata</i>
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
In order to establish and perfect ISSR-PCR reaction system of Pinellia ternata , we used P. ternata from Sichuan region as the test material in this research. Genomic DNA of whole plant of P. ternata was used as template, UBC818 primer was used in ISSR-PCR system, L 16 (4 5 ) orthogonal test and single factor experiment were used to optimize the system. Based on the visual analysis of orthogonal experiment, we found the following are the influences of various factors on the experimental results in turn: dNTPs, primer, Mg 2+, Taq DNA polymerase, DNA template. Based on single factor screening test, we found the best ISSR-PCR reaction system. The total reaction volume is 20.0 μL, containing dNTPs 0.2 mmol/L, primer 0.8 μmol/L, Mg 2+ 2.0 mmol/L, Taq DNA polymerase 0.075 U/μL, DNA 15 ng, annealing temperature 56℃. In this study, ISSR-PCR reaction system was optimized which will be applied to detect the genetic stability of P . ternata tissue culture seedlings.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.004 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it