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Record W4382930098 · doi:10.1101/2023.07.03.547316

Mechanical Force Imprints Neutrophils to Orchestrate Pulmonary Homeostasis

2023· preprint· en· W4382930098 on OpenAlexaff
Jin Wang, Haixia Kang, Wenying Zhao, Wenjuan Bai, Xin Qi, Kepeng Yan, Naijun Miao, Dong Dong, Yuanyuan Wang, Ajitha Thanabalasuriar, Dachuan Zhang, Youqiong Ye, Tian‐Le Xu, Bin Li

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2023
Typepreprint
Languageen
FieldMedicine
TopicErythrocyte Function and Pathophysiology
Canadian institutionsMcGill University
FundersSchool of Medicine, Shanghai Jiao Tong UniversityShanghai Jiao Tong UniversityNational Natural Science Foundation of China
KeywordsPIEZO1HomeostasisMechanosensitive channelsImmune systemLungBiologyCell biologyImmunologyAngiogenesisPopulationInflammationMedicineCancer researchInternal medicineIon channelReceptor

Abstract

fetched live from OpenAlex

Abstract Neutrophils are the most abundant immune cells that constantly patrol and marginate into the vascular beds of multiple tissues to support immune homeostasis. The extent to which neutrophils undergo adaptation in response to diverse tissue microenvironments, and the resultant biological implications of such adaptation, remains unclear. Here, we performed intravital imaging, transcriptional, and functional analyses of neutrophils in different tissues. Our findings showed that the lung harbors a transcriptionally distinct neutrophil population with unique migratory behaviors. These resident-like neutrophils guarantee a rapid response upon infection and play an important role in maintaining the homeostasis of pulmonary vasculature. Notably, pulmonary neutrophils are imprinted by mechanical cues via the mechanosensitive ion channel Piezo1. Mice with conditional Piezo1 ablation lost lung-specific neutrophil signatures, and showed impaired lung capillary angiogenesis. Furthermore, these mice displayed increased susceptibility to airway infection. Collectively, these data identify mechanical sensing via Piezo1 as an important driver for tissue-specialized neutrophils that support pulmonary homeostasis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.444
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.001
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0000.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.036
GPT teacher head0.262
Teacher spread0.226 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2023
Admission routes1
Has abstractyes

Explore more

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