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Record W4383107436 · doi:10.51161/bacterion/19414

COMPARAÇÃO DE DOIS SISTEMAS MATRIX-ASSISTED LASER DESORPTION IONIZATION - TIME OF FLIGHT / MASS SPECTROMETRY PARA IDENTIFICAÇÃO DE BACTÉRIAS ISOLADAS DE UMA INDÚSTRIA PRODUTORA DE IMUNOBIOLÓGICOS DO RIO DE JANEIRO

2023· article· pt· W4383107436 on OpenAlexaff
Maria Luiza Soares de Souza, Luciana Veloso da Costa, MARCELO LUIZ LIMA BRANDÃO, NATHÁLIA GONÇALVES SANTOS CALDEIRA, Viviane Zahner

Bibliographic record

Venuenot available
Typearticle
Languagept
FieldBiochemistry, Genetics and Molecular Biology
TopicIdentification and Quantification in Food
Canadian institutionsDiscovery Air (Canada)
Fundersnot available
KeywordsMass spectrometryPhysicsTime-of-flight mass spectrometryDesorptionAnalytical Chemistry (journal)ChemistryIonizationChromatographyIonPhysical chemistry

Abstract

fetched live from OpenAlex

INTRODUO:A identificao de microrganismos isolados no controle microbiolgico de produo farmacutica fornece informaes importantes para rastrear as fontes de contaminao, implementar aes corretivas adequadas, e compreender a composio da microbiota.A utilizao de mtodos de identificao rpidos e eficazes pode evitar o descarte de lotes sem necessidade ou liberao de produtos sem qualidade.Estudos utilizando a metodologia Matrix-Assisted Laser Desorption Ionization -Time of Flight / Mass Spectrometry (MALDI-TOF/MS) amostram-se como uma boa opo para a identificao de linhagens isoladas em indstrias, pela rapidez e pela acurcia dos resultados.A tcnica MALDI-TOF/MS rpida, mostra uma elevada especificidade e uma excelente sensibilidade.OBJETIVOS: O objetivo deste estudo foi comparar dois sistemas MALDI-TOF/MS na identificao de linhagens bacterianas isoladas a partir de uma indstria farmacutica produtora de imunobiolgicos localizada no Rio de Janeiro/RJ.METODOLOGIA: Foram utilizadas 63 linhagens isoladas no perodo de 2010 a 2021, cultivadas em gar Triptona de Soja, que foram analisadas nos equipamentos MALDI Biotyper (Bruker) e VITEK MS (bioMrieux), de acordo com

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesInsufficient payload (model declined to judge)
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.078
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0030.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.002
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0010.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.031
GPT teacher head0.304
Teacher spread0.273 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; both teacher heads agree on what is shown here.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

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