The Complete Genome Sequence of Clade B, Wheat StreakMosaic Virus Isolate from Turkey
Bibliographic record
Abstract
Abstract Wheat streak mosaic virus is one of the most widespread viruses in cereal crops, causing severe losses, dramatically affecting worldwide wheat production. Currently, four distinct clades of WSMV have been grouped and named: A (Mexico), B (Europe, Asia, Russia), C (Iran), and D (United States, Argentina, Brazil, Australia, Canada, Turkey). Each of these groups is based on genome-wide variability, emphasizing the CP. Previously reported Turkish wheat isolates of WSMV clustered within both clades D and B. However, the placement of the Turkish WMSV into clade B is only based on a partial genome sequence. Here, we used high throughput sequencing to assemble the complete genome sequence of WSMV type B isolate collected from wheat found in the European part of Turkey. Excluding the poly(A) tail, the genome of isolate S34Edirne (Genbank no. MZ405098) consists of 9,360 nucleotides and contains a single large open reading frame encoding a polyprotein of 3,033 amino acids. The characteristic lack of a GAG (Gly2761) codon within the CP of the polyprotein is typical for the clade B, WSMV-ΔE isolates, which are widely found throughout the European continent. However, two American isolates were recently placed in this group. Sequence comparisons revealed that WSMV Turkish wheat isolate is the most closely related to Czech isolate, with highly similar nucleotide and amino acid identities at 98.83-99.13%, respectively. The result of this study indicates that the WSMV full-length genome of S34Edirne isolate should be placed into clade B of the European WSMV-ΔE isolates.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".