Cloning, Bioinformatics and Expression Analysis of <i>SmWRKY53 </i>Gene in <i>Solanum melongena </i>L
Bibliographic record
Abstract
In this study, SmWRKY53 gene was selected from the data of high temperture transcriptome and gene expression detection of "Tewangda" seedlings. It was then cloned, bioinformatics and preliminary expression analyzed. The results showed that the open reading frame of SmWRKY53 gene was 1 083 bp, encoding 360 amino acids. The 138-144 amino acids contain a typical "WRKYGQK" conserved domain, and the zinc finger structure is C 2 HC type, belonging to the Group Ⅲ subfamily. The homology comparison and phylogenetic tree analysis showed that SmWRKY53 was closely related to StWRKY53 of potato ( Solanum tuberosum ), and the homology is 91.41%. Bioinformatics prediction showed that SmWRKY53 was a basic and unstable hydrophilic protein with no signal peptide and transmembrane domain. The ratio of random coil in the secondary structure of protein was the highest, 63.89%. There was amino acid disorder structural regions in the SmWRKY53 protein. Subcellular localization prediction indicated that the protein was most likely located in the nucleus. Quantitative Real-time PCR results showed that the relative expression levels of SmWRKY53 was the highest in mature stem and the lowest in flesh. The expression of SmWRKY53 in roots, stems and leaves of eggplant seedlings first increased and then decreased, and reached the peak at 3 h under high temperature stress. This study provides the reference for further exploring the function of SmWRKY53 gene.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".